Rahul Nimba Patil

Background: Inflammation is the immune response to harmful stimuli, such as pathogens, damaged cells, toxic compounds, or irradiation and acts by removing injurious stimuli and initiating the healing process. Inflammation is therefore a defense mechanism that is vital to health. Usually, during acute inflammatory responses, cellular and molecular events and interactions efficiently minimize impending injury or infection. Current herbal medicine has been found effective with minimal or no side effect. Materials and Methods : Polyherbal extract of plants which contains curcumin, withanolides, withanoferins, linoleic acid are the active constituents responsible for Anti-inflammatory effect. Extraction of the plant material was done to obtain extract and further proceed for physical characterization and phytochemical analysis. TLC was done to confirm the active constituents present in polyherbal with reference to standard. The polyherbal extract was compared with standard to evaluate the anti-inflammatory by in-vitro assessment of Bovine albumin fraction and Protein denaturation assay. Result and Conclusion : The percentage yield of extracts were found to 66.7, 74.2, 63.7. The Rf value of polyherbal extract was found to be 0.82 which matches with the standard and hence presence of curcumin, withanolides, withanoferins, linoleic acid was confirmed. The dose dependent study and statistical comparison with the help of graph pad prism, version 9.03 by one way ANOVA followed by dunnett’s multiple comparison test has revealed that moderate inhibition (p≤0.05) is observed in albumin and protein denaturation treated with Polyherbal extract when compared with standard Diclofenac sodium and hence the Anti-inflammatory effect of Polyherbal extract is justified.

ackground: Mammary cancer is the most common type of cancer and the leading cause of cancer-related death in women. Despite of numerous therapeutic options, cancer remains associated with high mortality. Traditional herb medicine has been found effective with minimal or no side effects. Materials and Methods: The leaves of Dolichandrone falcata Seem. (Bignoniaceae family) contains chrysin-7-rutinoside, flavanoids which possess Anti-cancer potential. The extract obtained from Soxhlet extraction process is then assessed against the chemical carcinogen (7,12-Dimethyl benzene anthracene) induced mammary carcinoma in rats. Results and Conclusion: The dose dependent study and statistical comparison with the help of graph pad prism, version 8.03 by one way ANOVA followed by Dunnett’s multiple comparison test has revealed that the significant reduction (p

Background:Diabetes mellitus is very common metabolic disorder which affects the human population throughout the world, characterized by hyperglycaemia and arises due to defects in insulin secretion, insulin action or both. Despite of numerous therapeutic options, Diabetes mellitus remains associated with high rate. Traditional herbal medicine has been found effective with minimal or no side effect.Dolichondrone falcata. Seem Described in Ayurveda as a significant therapeutic herb, Seem is a member of the Bignoniaceae family. The herb has a variety of phytochemical compounds, which have been linked to a number of pharmacological effects, including Abortive, Anti-inflammatory, Anti-cancer, Anti-diabetic, Antioxidant, Anxiolytic, Analgesic, and Immunomodulatory etc. Dolichondrone falcata. Seemfruit have been noted to have Anti-diabetic properties. Dolichandrone Falcata. Seem contained phytochemical constituents such flavonoids, tannins, glycosides, and steroids, according to a phytochemical examination. Recent phytochemical analysis of Dolichondrone falcata. Seem fruit and leaves revealed the presence of alkaloids such barberin, catharanthine, and aegelin, which are actively known for reducing blood sugar levels.Therefore, current research was carried out to assess the Anti-diabetic potential of aerial parts of Dolichandrone falcata. Seem extract. Materials and Methods:The aerial parts of Dolichandrone falcata.Seem revealed that it was a good source of barberin, catharanthine, and aegelin phytochemical constituents are well known for their lowering the blood sugar level and which posses Anti-diabetic potential. The extract of aerial parts of Dolichandrone falcata.Seem is assessed against enzyme alpha amylase and alpha glucosidase for inhibition. Results and Conclusion: The dose dependent study and statistical comparison with the help of graph pad prism, version 9.03 by one way ANOVA followed by Dunnett’s multiple comparison test has revealed that significant inhibition (p≤0.05) is observed in alpha amylase and glucosidase enzyme treated with Dolichandrone falcata. Seem extractswhen compared with standard Acarbose and hence the Anti-diabetic potential Dolichandrone falcata. Seem extracts is justified.

Background: When the kidneys are unable to eliminate toxic substances (impurities or wastes) from the blood, hemodialysis is a viable option. Hemodialysis is a continuous process that takes approximately 4 hours to complete. Unlike normal kidneys, it cannot continuously monitor body functions. However, after a hemodialysis session, it can remove waste products and restore pH and electrolyte levels. Numerous changes in the fluid and mineral compartment occur during the procedure, which causes patients to experience a range of complications ranging from minor to potentially fatal. The study's objectives are to assess and analyse complications that arise from hemodialysis as well as to ascertain the occurrence of such complications. Methods: This was a prospective study. A data collection form was used to collect demographic data, surgery, comorbidity and the self-design severity scale to note during hemodialysis. Results: Of the 190 patients in total, 70 (36.6 %) were female and 120 (63.15 %) were male. In 166 (88 %) of the patients who were interviewed, the venous access used for hemodialysis was an arterio venous fistula (AVF). F6HPS was administered as the dialyzer to 82 patients (43.15 %). Our self-made assessment tool revealed that 141 patients (74.21 %) had severe complications, 23 patients (12.105 %) had moderate complications, and 30 patients (15.78 %) had mild complications. In a different investigation, [Mehmood et al.,2016] 12.5 % of patients experienced cramps, and 37.5% experienced hypotension. In our study, cramps affected 18 patients (28 %) and hypotension affected 25% of patients as an intra dialytic complication. The primary complications in our study involving patients with AKI and CKD were these two issues. In our study, there is no discernible relationship between age and sex distribution. Conclusions: According to our study, it is concluded that hypotension (47.8 %), muscle cramps (43.6 %), and hypertension (30.5 %) occur commonly and necessary precautionary measures has to be kept in line, if in case an emergency occur. Fever (3.6 %), hypoglycemia (1.57 %), nausea and vomiting (12.6 %). Significant relationship was seen between complications and age and renal failure in contrasting to it no significant relationship not seen between sex and type of access.

The anthelmentic impact of Dolichondrone falcata. seem leaves was assessed using adult Pheretima Posthuma Indian earthworms. Earthworms were used to test various ethanolic extract concentrations, ranging from 10 to 100 mg/ml. These Dolichondrone falcata. seem leaves ethanolic preparations were assessed for bioassays, which included the worms' times of paralysis and death. At a dosage of 100 mg/ml, ethanolic extract demonstrated a potent anthelmentic activity. Drug concentrations that were lower did not have a discernible anthelmentic impact. As the standard reference medication, piperazine citrate at a concentration of 10 mg/ml was utilised. Keywords: Dolichondrone Falcata, Pheretima Posthuma, Anthelmentic

Background: Breast cancer is the most commonly diagnosed malignancy in women and a leading cause of cancer-related mortality worldwide. Despite advancements in diagnostic and therapeutic strategies, breast cancer remains a significant clinical challenge due to its heterogeneity and the adverse effects of conventional treatments. The use of traditional herbal medicine, known for its minimal side effects and efficacy, offers a promising alternative. Material & Methods: Aerial parts of Anethum graveolens seeds and Spinacia oleracea leaves were extracted using the Soxhlet extraction method. This study investigates the anti-mammary carcinoma potential of Anethum graveolens (dill) seeds and Spinacia oleracea (spinach) leaves, known for their bioactive compounds with anti-cancer properties. Result & Discussion: At the different doses (10 to 100 µg/ml) of polyherbal extracts carried out for anticancer activity against MCF-7 cell line., the polyherbal extracts showed good activity against breast cancer cell line when compared with the standard drug 5 FU. These findings highlight the anti-cancer potential of polyherbal extract of Anethum graveolens seeds and Spinacia oleracea leaves as natural therapeutic agents for the management of mammary carcinoma.

Background: Diabetes mellitus is very common metabolic disorder which affects the human population throughout the world, characterized by hyperglycaemia and arises due to defects in insulin secretion, insulin action or both. Despite of numerous therapeutic options, Diabetes mellitus remains associated with high rate. Traditional herbal medicine has been found effective with minimal or no side effect. Materials and Methods: The aerial parts of Momordica charantia, Embilica officinialis, Trigonella Foenum-graecum, Murraya koennigii, Azadirachta indica, Allivum sativum contains charantin, vicine, polypetide-P, triterpenoids which posses Anti-diabetic potential. Extraction of the plant material was done to obtain extract and further proceed for physical characterization and phytochemical analysis. TLC was done to confirm the active constituents present in polyherbals with reference to standard. The extract obtained from various extraction process is mixed in together in equal quantity to prepare polyherbal extract and assessed against enzyme alpha amylase and alpha glucosidase for inhibition. Results and Conclusion: The % yield of the extract was found to be 69.2, 72.8, 58.8. The TLC has identified the flavonoids and triterpenoids named Charantin, Picine and Polypeptide-p in the polyherbal extract 1 and 2. The Rf value of polyherbal extracts was found to be 0.78 and 0.76 which matches with the standard and hence presence of Charantin, Vicine and Polypeptide-p was confirmed. The dose dependent study and statistical comparison with the help of graph pad prism, version 9.03 by one way ANOVA followed by dunnett’s multiple comparison test has revealed that significant inhibition (p≤0.05) is observed in alpha amylase and glucosidase enzyme treated with Polyherbal extract 1 and 2 when compared with standard Acarbose and hence the Anti-diabetic potential potential of Polyherbal extracts is justified. Keywords: Diabetes mellitus, Polyherbal extract, Anti-diabetic, Alpha amylase, Alpha Glucosidase.

This study explores the comparative anti-cancer potential of Aegle marmelos Linn. (Bael) seed and bark extracts against MCF-7 human breast cancer cell lines using in-vitro assays (MTT and SRB). Extracts were prepared using ethanol and water and evaluated for phytochemical constituents, physical parameters, and cytotoxic efficacy. Phytochemical analysis confirmed the presence of alkaloids, tannins, saponins, and flavonoids. The ethanolic bark extract demonstrated a significant dose-dependent cytotoxic effect, achieving an IC₅₀ of 82.17 µg/ml in the MTT assay and 44.03 µg/ml in the SRB assay. In contrast, the aqueous seed extract showed comparatively lower activity. These findings suggest that the ethanolic bark extract of Aegle marmelos exhibits notable anti-cancer activity and has the potential to be developed into a plant-based therapeutic agent for breast cancer treatment.

Background: Considering the prevalence of epilepsy and the problems Salvia officinalis Linn., associated with currently available anti-epileptic drugs like side effects, resistance, safety issues and high cost, herbal medicines with fewer complications could be very appropriate alternative. Therefore, the present study was examined for the anti-epileptic activity of ethanolic extract of Salvia officinalis Linn. stem. Material and Methods: Earlier studies suggested that Phyto-constituents like flavonoid, alkaloid and tannins shows anti-epileptic activity and comprehensive literature revealed that Salvia officinalis is rich in flavonoids like chlorogenic acid, ellagic acid, quercetin, rosmarinic acid, as well as the several volatile components such as borneol, cineole, camphor, and thujone, alkaloids, tannins, polyphenol, saponins etc. Anti-epileptic activity of Salvia officinialis. Linn stem in mice was evaluated using Pentylenetetrazole (PTZ) induced seizures, Strychnine induced convulsion and Picrotoxin induced convulsion GABA estimation method. Result: The ethanolic extract at 100 mg/kg, 200 mg/kg and 400 mg/kg possess dose dependent anticonvulsant activity as it reduced the duration of seizures produced by and latency of seizures produced by PTZ, Strychnine, and Picrotoxin. The amount of GABA estimation which is most likely to be involved in seizure activity was increased in mice brain and the anti-epileptic activity of ethanolic extract of Salvia officinalis Linn. stem is justified.