Authors : Sharlee R, Sharlee R, Sumangala B, Sumangala B
DOI : 10.18231/j.ijmr.2020.040
Volume : 7
Issue : 3
Year : 2020
Page No : 222-225
Background: Bioflim is a mode of survival for various microbe by which they form aggregates during
unfavourable conditions. Staphylococcus aureus is a major cause of nosocomial and community acquired
infections.
Materials and Methods: A total of 150 Staphylococcus isolates were screened for biofilm production by
Congored method and Tube method following standard guidelines.
Results: Of the 150 isolates, 85(56.6%) were MSSA and 65(43.4%) were MRSA. On Congored agar, 63
isolates showed black colonies with dry crystalline consistency indicating biofilm production. Out of 63
isolates 62% of isolates were MRSA and 38% of isolates were MSSA. Biofilm by tube method, 84isolates
showed biofilm production. MSSA were 48.2% and MRSA were 81.53%.
Conclusion: MRSA is the significant biofilm producer when compare to MSSA, Congored method is less
accurate when compare to tube method as screening test for the detection of biofilm.
Keywords: MRSA-Methicillin resistant Staphylococcus aureus, MSSA- Methicillin sensitive Staphylococcus aureus, Biofilm, Congored.