Antibiotic susceptibility patterns of Pseudomonas sp. isolated from various clinical samples at a tertiary care hospital at Dewas in Madhya Pradesh, India
Background: A significant proportion of nosocomial infections is contributed by the Pseudomonas sp. Major changes were observed from time to time in Pseudomonas sp. prevalence and multidrug-resistant (MDR) with enormous morbidity and mortality. The spread of antimicrobial resistance has now become a worldwide issue as a consequence of the haphazard use of antimicrobials.
Place and duration: This study was done in the Amaltas Institute of Medical Sciences from February 2019 to December 2019.
Materials and Methods: Pseudomonas sp. was isolated from different clinical samples like urine, sputum, pus, fluid, and catheter tip. Identification of Pseudomonas sp. was done by biochemical tests. Antibiotic susceptibility test (AST) was done by Kirby-Bauer disc diffusion method. Data Analysis was done by percentage method. Extended-spectrum β-lactamase (ESBLs) production was detected by the combined disc diffusion test.
Results: Sensitivity was seen in colistin and polymyxin B - 100%, meropenem - 86.58% and piperacillin -tazobactam - 82.92%. Resistance was seen in co-trimoxazole- 80.48%, cefixime- 82.92%, cefoperazone/sulbactam-59.75% and ciprofloxacin- 39.02%. The maximum number of MDR strains were
isolated from the pus samples (n=18) as compared to other samples. The total number of ESBL producing strains was seen higher in the pus samples (n=15). The number of P. aeruginosa strains (n=57) was seen greater in number than other Pseudomonas sp.
Conclusion: The present study is an attempt to regulate the use of antibiotics that are irrelevant and wasteful. It is also a public health concern to take adequate measures, maintain cleanliness and hygiene to reduce the spread of nosocomial infections.
Keywords: Antibiotic susceptibility test (AST), Kirby-Bauer disc diffusion method, Pseudomonas aeruginosa, Multiple drug resistance (MDR), Extended-spectrum β-lactamase (ESBL)
Place and duration: This study was done in the Amaltas Institute of Medical Sciences from February 2019 to December 2019.
Materials and Methods: Pseudomonas sp. was isolated from different clinical samples like urine, sputum, pus, fluid, and catheter tip. Identification of Pseudomonas sp. was done by biochemical tests. Antibiotic susceptibility test (AST) was done by Kirby-Bauer disc diffusion method. Data Analysis was done by percentage method. Extended-spectrum β-lactamase (ESBLs) production was detected by the combined disc diffusion test.
Results: Sensitivity was seen in colistin and polymyxin B - 100%, meropenem - 86.58% and piperacillin -tazobactam - 82.92%. Resistance was seen in co-trimoxazole- 80.48%, cefixime- 82.92%, cefoperazone/sulbactam-59.75% and ciprofloxacin- 39.02%. The maximum number of MDR strains were
isolated from the pus samples (n=18) as compared to other samples. The total number of ESBL producing strains was seen higher in the pus samples (n=15). The number of P. aeruginosa strains (n=57) was seen greater in number than other Pseudomonas sp.
Conclusion: The present study is an attempt to regulate the use of antibiotics that are irrelevant and wasteful. It is also a public health concern to take adequate measures, maintain cleanliness and hygiene to reduce the spread of nosocomial infections.
Keywords: Antibiotic susceptibility test (AST), Kirby-Bauer disc diffusion method, Pseudomonas aeruginosa, Multiple drug resistance (MDR), Extended-spectrum β-lactamase (ESBL)