AbstractIntroduction: Orthodontic tooth movement causes sequential release of numerous biomarkers from the periodontal tissues, this study helps us to better understand the biological processes involved.
Objectives: This study was designed to detect and assess the Gingival Crevicular Fluid (GCF) levels of lysosomal protease, Cathepsin K during human orthodontic tooth movement.
Materials and Methods: Eight bimaxillary protrusion patients undergoing orthodontic treatment with four first bicuspid extractions were selected. Retraction of the canine was initiated by giving lace back on maxillary right canine which was used as Control Tooth (CT) and maxillary left canine used as Test Tooth (TT) with no laceback. From each patient 4 GCF samples were collected 1 hour before, on 1st day (24 hours), on 7th day(168 hours), and after 1 month(30 days). The dynamics of mechanically stimulated Cathepsin K levels in GCF was assessed using enzymatic immunoassay (ELISA).
Results: Results show significant differences between the control and treated teeth for Cathepsin K, with mean values significantly higher for treated site than control sites. On 7th day, at the test side, the levels of Cathepsin K were higher than the corresponding control sides. Another important finding was seen on the 30th day, where Cathepsin K levels were significantly higher on the control side when compared to the test side.
Conclusion: These results indicate that the amount of Cathepsin K in GCF increased during the initial period of orthodontic tooth movement. However, levels of Cathepsin K can be reliably measured during the acute phase of treatment and beyond that the values are unreliable.
Keywords: Cathepsin K, GCF, ELISA.
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