Authors: Jyot Amrita, Mridula Mahajan
Introduction: Cardiovascular disease (CVD) is a serious threat in female population particularly after menopause. The prevalence of CVD with associated risk factors in Punjabi population after menopause is rapidly increasing. Adiponectin polymorphism ADIPOQ +276G/T is located in intron 2 which results from G to T substitution. The variants of adiponectin gene are found to be associated with obesity, metabolic syndrome markers and cardiovascular disease. Aim: The present study aimed to screen the prevalence of +276 G/T polymorphism of AdipoQ gene in menopausal women of Punjab with and without the family history of CVD Materials and Methods: Genomic DNA was extracted from intravenous blood for all the participants. Genotyping of +276 G/T polymorphism in the AdipoQ gene was done by polymerase chain reaction (PCR) – based Restriction Fragment Length Polymorphism (RFLP). Results: Prevalence of TT genotype was higher among cases (22.3%) than among controls (4.4%). Statistical significant difference (p (p=0.020) in cases with positive family h/o CVD. TT genotype also provided significant risk ~9 folds towards CVD predisposition [p=0.001; OR=9.90(3.77-25.99)] in cases with negative family h/o CVD. Conclusion: The present study reveals that prevalence of TT genotype was found to be more in cases as compared to controls and also positive family history of CVD alone does not seem to be a good predictor as individual risk for cardiovascular disease in Punjabi menopausal women. Keywords: AdipoQ gene, +276 G/T polymorphism, Cardiovascular disease, Family h/o CVD, Menopausal women.
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