Vijaya Lakshmi D

Abstract

Aims: Epidemiology of Candidiosis their prevalence, identification and evaluation of extra cellular enzymes patterns in non albicans Candia group from the oral washings of diabetic subjects. Materials and Methods: A total of 125 oral samples were collected and analyzed. The Candiad species were identified by means of morphological and by molecular methods. The production of virulence factors assessed by using standard protocols. Results: Total of 70 different yeast like fungal strains were isolated and identified. The selected 6 virulence factors were investigated and compared critically among the NAC species. The overall observations were indicating that the virulence factors are fluctuated randomly with in the strains tested. Maximum extent of the virulence factors were reported as Phospholipase [Candida parapsilosis], protease [Candida tropicalis-2, Candida parapsilosis], Haemolysin [Candida tropicalis-3], Coagulase [Candida tropicalis-3], and Biofilm formation [Candida parapsilosis] respectively. Conclusions: In the present study we focused mainly on NAC species and their credible factors responsible for the progression of infections in diabetic subjects. Keywords: Virulence factors, Non albicans Candida, Hydrolytic enzymes, Diabetic subjects, Oral candidiasis.

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Abstract

Dipodascuscapitatus is a typical inhabitant of humans, especially on the skin, oral cavity, and respiratory tract. Fungi previously considered harmless colonizers (Opportunistic) are emerging as new fungal pathogens, particularly in an immune-compromised state. Infection caused by Dipodascuscapitatus is rare, and the treatment procedures are quite difficult. Here we reported the information on patients with chronic diabetic conditions. The fungus was isolated from oral washings, the phenotypic identification was based on mycological methods, the molecular marker was based on 18S r RNA sequencing, and the susceptibility test was conducted by micro-dilution technique. The present study mainly focused on quantifying virulence factors, and their activity is expressed in the form of hemolysin protease (1.812 mg/ml). Fungal infections in these patients are often severe, rapid progressive, and challenging to identify, including diagnostic and therapeutic modalities required to provide better patient care. Keywords: Non albicans Candida, Diabetics, Oral washings, Virulence factors, Drug resistance, Dipodascus capitatus

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