Shota Kepuladze

Abstract

Ovarian cancer represents one of the major causes of gynaecologic cancer mortality worldwide. Tumor-associated macrophages (TAM) are associated with the development and progression of ovarian cancer. TAMs are mainly represented by two types M1 and M2. We investigated the distribution of total, M1, M2 macrophages, tumor cell/macrophage ratio and M1/M2 ratio in mucinous and serous ovarian tumors. The study results showed that total TAM count is significantly higher in serous ovarian tumors, compared to mucinous tumors and the highest infiltration rate is detected in high-grade serous ovarian carcinomas. In addition, the number of M2 macrophages is significantly increased in higher-grade serous ovarian carcinomas. The evaluation of tumor/cell macrophage ratio could be used as an objective measure of macrophage infiltration in ovarian tumors.   Keywords: Tumor-associated macropages, Ovarian cancer, Tumor microenvironment.

View Article

Abstract

During recent years, there is an accumulating evidence that high grade ovarian carcinoma is developed from the fallopian tube epithelial lesions. However, is not yet completely understood and still represents the subject of investigation. We investigated the immunohistochemical phenotype of matched fallopian tubes from the patients with different types and malignancy grades of ovarian carcinoma. Matched fallopian tubes from ovarian cancer patients were available in 260 cases, including mucinous borderline tumor (n=25), mucinous carcinoma (n=15), serous borderline tumor (n=90), low grade serous carcinoma (n=72) and high grade serous carcinoma (n=48). Immunohistochemical investigation included markers of proliferation (Ki67), apoptosis (Bcl2, p53), hormone receptors (ER, PR), epithelial differentiation (CK7), mesenchymal differentiation (vimentin, calretinin) and stem cells (CD44). The results indicate that the presence of fallopian tube carcinoma in situ is significantly correlated with the presence of high grade ovarian serous carcinoma (r=0.44, p   Keywords: High grade ovarian carcinoma, Fallopian tubes.

View Article

Abstract

Epithelial lesions of the conjunctiva include both benign and transitional malignant and malignant lesions., Therefore, it is important to identify molecular markers of malignant progression of these lesions. The aim of our study was to study molecular markers determining the risk of progression of epithelial neoplastic processes of the conjunctiva. The following markers were detected by standard immunohistochemical methods: Ki67, PHH3, Bcl2, P16, HPV16/18, P53, P63 and CK7.A squamous-epithelial index was also derived by evaluating standard hematoxylin-eosin (H&E)-stained fluorescence and the P63/CK7 ratio. The results of the study showed that during the progression of intraepithelial neoplasia of the conjunctiva, the content of squamous epithelium and, accordingly, the squamous-gland index increases significantly. In addition, according to proliferative and apoptotic characteristics, conjunctival intraepithelial lesions can be divided into two groups, namely, low-grade intraepithelial neoplasia and high-grade intraepithelial neoplasia. Keywords: Conjunctival Neoplasms, Conjunctival Dysplasia, Conjunctival intraepithelial neoplasm, Conjunctival squamous cell carcinoma

View Article