Rumana Farooq Mir

Rumana Farooq Mir

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Profile ID RP-000-1747 Joined 2023-01-18 2 articles
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Authors: Bushra Yousuf Peerzada, Rumana Farooq Mir, Lubna Samad, Aliya Shah

DOI: 10.18231/j.ijmmtd.2022.015  

Volume8

Issue1

Pages69-72

Abstract

Introduction: Intestinal parasitic infections represent a grave public health problem especially in developing nations like India, leading to malnutrition, growth retardation, anaemia’s and vitamin deficiencies in early childhood. As such the burden of these intestinal parasitic infections in the society needs to be focussed at the right time which will in turn lead to enhanced health and improved economic conditions of the country. Aim: To find out the prevalence of intestinal parasitic infections in a tertiary care hospital in Kashmir. Materials and Methods: A retrospective study was carried out in the Parasitology division in the department of Microbiology of Government Medical College, Srinagar for a period of five years. Routine stool examination was studied to detect the parasitic infections among the patients attending various outpatient departments of our hospital. Results: A total of 2159 stool samples were examined in five years out of which 165 (7.6%) revealed the presence of parasites. The most common parasite identified was (71.9%) followed by Giardia lamblia (16.4%). Conclusion: Intestinal infection due to was the most common parasite identified in our study. The prevalence of these infections can still be higher as these parasites are excreted intermittently in stool samples. As such taking repeat samples from same patients suffering from intestinal parasitic infection is important followed by concentration methods, which together will help and enhance better retrieval of intestinal parasites reflecting the total burden of these infections in our community. Keywords: Ascaris lumbricoides, Iodine wet mount, Intestinal infection

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Authors: Rumana Farooq Mir, Bushra Yousuf Peerzada, Lubna Samad

DOI: 10.18231/j.ijmmtd.2020.053  

Volume6

Issue4

Pages237-242

Abstract

Introduction: With the surge of multidrug-resistant tuberculosis (MDR-TB) strains there is an escalating need for precise and cost-effective methods for a precipitous diagnostic and drug susceptibility testing (DST), chiefly in resource limited countries where tuberculosis is endemic. Aim: To find out the susceptibility pattern of Isoniazid (INH) and Rifampicin (RMP) in smear positive cases of pulmonary TB at MMIMSR Mullana and to evaluate the sensitivity and specificity of nitrate reductase assay (NRA) with standard proportion method for INH and RMP susceptibility testing.  Material and Methods: A cross sectional test validation study was carried out at Mycobacteriology division in the Department of Microbiology on 100 smear positive sputum samples from pulmonary tuberculosis patients. Results: In comparison with L.J proportion method, NRA showed sensitivity, specificity, PPV, NPV, accuracy of 100%, 98.64%, 87.50%, 100%, and 98.76% respectively for INH. The sensitivity, specificity, PPV, NPV, accuracy of NRA compared to PM for RMP was 100%, 97.33%, 75.00%, 100%, 97.53% respectively. Conclusion: Direct NRA is simple, easy to perform, prompt, reasonably less expensive, without requisite of expensive reagents and advanced instrumentation. It is highly specific and sensitive technique in the detection of drug resistant TB when tested in parallel with the proportion method. Therefore, NRA may be routinely employed in TB laboratories in developing countries for drug susceptibility testing of M. tuberculosis. Keywords: Tuberculosis, Multidrug resistant, Nitrate Reductase Assay.

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