Sreenivasa Charan Archakam

Abstract

Aim: The aim of the present is to develop simple, accurate and precise RP-HPLC method for the quantification of Eugenol content in various tulsi based ayurvedic formulations. Materials and Methods: HPLC was carried out by reverse –phase technique on a C18 column with a mobile phase composed of Acetonitrile: Methanol in the proportion of 50:50 v/v, at a flow rate of 1.0 mL /min. The detection was carried at 281 nm by UV- Visible detector. The linearity was established in the concentration range of 10-50 mcg/mL with an R value of >0.999. Method and system precision was established with an RSD of < 2> Results: The method was found to be specific, precise, accurate and robust. The proposed method can be used for the reliable quantification of Eugenol. Keywords: Eugenol, Tulsi, RP­HPLC, Accuracy, Precision, Ayurvedic formulations

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Abstract

Aim: The present study aimed to develop simple, accurate and precise FTIR and UV spectrophotometric methods for the quantification of Atenolol and Hydrochlorothiazide in bulk and tablet dosage forms. Matrials and Methods: FT-IR method like classical least squares (CLS) was developed within the range of 2366.69-3433.44; 1564.40-1673.30 cm- UV methods like Cramer’s matrix method (method-I) and linear regression analysis (Method II) were developed and they are based upon constructing the matrix set by using molar absorptivity values at 275.60 nm and 270.40 nm. Results: The assay values for FTIR- CLS method were 102% and 108 % for Atenololand Hydrochlorothiazide respectively. Cramer’s matrix method results were found to be 95.15% and 104% for Atenolol and Hydrochlorothiazide respectively and for linear regression method they were found to be 98.50% and 106% (w/w).   Keywords : Classical least squares (CLS), Cramer&#39;s matrix method, Linear regression analysis

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