Sneha Aditi

Abstract

Background: Benign prostatic hyperplasia (BPH) and prostate adenocarcinoma are the most common disorders affecting elderly men. The diseases are androgen-dependent and are treated by obstructing androgen receptor (AR) or their action. AR has direct relationship between the development of prostatic hyperplasia and prostatic carcinoma. Estrogens directly target prostate tissue by definite estrogen receptors (ER). The human prostate is primed with a dual system of ERs (ER-? and ER-β). Use of estrogens as hormonal agents is for suppressing prostatic tissue growth. Study aims to identify, assess and establish the significance of ER and AR as a marker in distinguishing BPH and prostatic carcinoma. Materials and Methods: A cross sectional study was conducted at a tertiary care hospital over a period of two year on 65 specimens which included 30 malignant cases and 35 benign cases. Specimens received were tru-cut biopsy, transurethral resection of prostate chips (TURP) and prostatectomy specimen for malignant cases and TURP for benign lesions. Paraffin embedded blocks was stained with H&E and IHC with ER and AR performed on representative sections and graded .the results were assessed for association with various parameters in both benign and malignant cases. Results: Out of 30 cases of adenocarcinoma of prostate majority of specimens were needle biopsies (54%). The age of presentation was 55-84 years with mean age being 68.93 years. In cases of BPH the presenting age was 35-84 years and mean age was 63 years. Histopathological parameters assessed for malignant lesions, showed negative correlation of grade group with PNI and positive correlation with neutrophils. Higher grade showed adverse histological parameters. %PSA was Conclusions: Hormones play a multifactorial role in the pathogenesis of BPH and prostatic carcinoma; most commonly androgen and estrogen, and can be used towards finding a better therapy. Keywords: Prostatic adenocarcinoma, BPH, Androgen Receptor, Estrogen Receptor, Grade group, Gleason's score

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Abstract

Background: SARS-CoV-2 causing corona virus disease 2019 (COVID-19) is the most common infection in recent days worldwide. Rapidly evolving from the epidemic outbreak in Wuhan, China on 29 December 2019, SARS-CoV-2 is the third known corona virus that causes fatal respiratory diseases in humans. Aim: The roles of biomarkers from a blood sample in the diagnosis of COVID-19 patients who have visited the hospital with history of fever has been evaluated. Materials and Methods: A retrospective study was carried out in a private sector hospital at Panipat where the patients having history of fever were admitted during 1st May 2020 to 15th August 2020. A total of 100 patients were included who were suspected cases of corona. All the blood parameters, systemic inflammatory index (SII), neutrophil lymphocyte ratio (NLR) and platelet lymphocyte ratio (PLR) Coagulation factor (prothrombin time (PT), activated partial thromboplastin time (aPTT), international normalized ratio (INR) and C-Reactive protein (CRP) are compared between negative and positive COVID-19 cases according to RT-PCR. Result: Out of 100 patients, 65% were RTPCR covid positive. In patient with RTPCR negative test results, it was found that total leukocyte count, neutrophils, platelet, NLR and SII values were higher. However, haemoglobin, lymphocytes and PLR was found to be elevated in patients with positive test results. Discussion: COVID-19 positive patients presented with leukopenia, neutropenia, thrombocytopenia and lymphocytosis. SII and NLR were reduced. PLR and CRP were increased. PT and APTT were within normal limits. Lymphocyte count and PLR showed strong significance in this study. Conclusion: The definitive diagnosis of COVID-19 was made by RT-PCR analysis, but this is a time-consuming and less accessible test. Low values of NLR and SII and high value of PLR and CRP are indicative of COVID-19 and can be used in its diagnosis.   Keywords: SARS- CoV- 2, RT-PCR, Systemic inflammatory index, Neutrophil lymphocyte ratio, Platelet lymphocyte ratio, Coagulation factor, Coronavirus

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