Influence of immunogen count to antibody response of inactivated avian influenza virus oil based vaccine in broilers

Authors : Sadia Zia, Muhammad Danish Mehmood, Huma Anwar, Saba Gul, Ejaz Rafique

DOI : 10.18231/2394-5478.2019.0010

Volume : 6

Issue : 1

Year : 2019

Page No : 44-49

Primary prerequisite of effective influenza virus immunization is homology of vaccinal strain to the wild type and appropriate immunogen count in each dose of vaccine to induce protective immune response. The purpose of current project is to study the effect of immunogen potency in inactivated monovalent and bivalent avian influenza vaccines. Eight AIV (Monovalent & Bivalent) oil based vaccines with different immunogen level were prepared and evaluated for potency in AIV susceptible broilers through Haemagglutination Inhibition (HI) test on 18 and 36-day post vaccination. It was observed that 0.3ml dose of inactivated avian influenza oil based AIV-H9 vaccine having infectivity titer EID₅₀ 1×10^9.2/ml and (HA= 512) induced high mean anti AIV-H9 antibody titers (72±22.62) as compared to the vaccine containing EID₅₀ 1×10^8.2/ml and (HA= 256). Similarly, same dose of bivalent avian influenza inactivated vaccine containing infectivity titer EID₅₀ 1×10^9.2/ml and (HA= 512) induced better immune response against each antigen H7 and H9 respectively, that of 0.3 ml dose of the vaccine comprising infectivity titer EID₅₀ 1×10^8.2/ml and (HA= 256) where H7 and H9 were documented. It was concluded that infectivity titers in 0.3 ml dose of inactivated adjuvanted vaccine shall be ? 10^8.2 /ml or 256 HAU to achieve protective anti body titers to get protection against avian influenza virus and its complications over a long period of time thus contributing to disease control in epidemics.

Keywords: Haemagglutination (HA), Infectivity titer (EID₅₀), Avian influenza virus (AIV).

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