Authors : Rudi Emerson De Lima Procopio
DOI : 10.18231/j.ijmmtd.2020.043
Volume : 6
Issue : 3
Year : 2020
Page No : 191-192
The selection of epitopes has become easier and faster with the aid of bioinformatics. SARS-CoV-2 is a single-stranded positive RNA virus (+ ssRNA) with approximately 30 kilobases, containing four main structural proteins: spike glycoprotein (S); small envelope protein (E); membrane protein (M); and nucleocapsid protein (N), which can generate epitopes. The protein chosen in this study was membrane glycoprotein ORF5, with 669 nt forming 222 amino acids. The analysis of the interaction of the protein
with the membrane showed that most of the protein is inside the viral envelope and approximately 25 amino acids outside, where the initial and final regions of the protein are ideal for generating epitopes. The first 20 amino acids (MADSNGTITVEELKKLLEQW) were chosen because they are on the outer surface of the membrane of the viral envelope. For the production of heterologous protein in Escherichia coli, two expression systems (pET-28a and pFLAG-ATS) were used to carry out the various immunological tests necessary to validate the results obtained.
Keywords: SARS-CoV-2.