Authors : Amit Pandey, Ankita Srivastava, Niharika Chandra, Dheerendra Kumar, Sunil Kumar
DOI : 10.18231/2394-5478.2018.0040
Volume : 5
Issue : 2
Year : 0
Page No : 188-193
The search for new bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant (MDR) pathogens. In the present study, the antimicrobial potential of new bacterial species has been evaluated by initial screening of fourteen soil samples. Primary and secondary screening was performed against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Four active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. One most active microbial strain was identified using 16S rRNA sequence homology and designated as Bacillus cereus (C13). Furthermore the production of secondary metabolites was done in optimized production media and the analysis involved HPLC method to identify metabolites based on retention time where 2.833 min retention time obtained using C18 column at 265 nm wavelength, which is similar to valinomycin antibiotic which is also secreted by Streptomyces griseus. It shows presence of bioactive secondary metabolites which is responsible for antibacterial activity of bacterial isolates.
Keywords: Antimicrobial activity, Actinomycetes, Methanolic extract, Minimum inhibitory concentration, Streptomyces