Sumaira Qayoom

Abstract

Purpose: To study the biofilm formation and to examine the correlation between antibiotic resistance and biofilm formation among the clinical isolates of Acinetobacter. Materials and Methods: A total of 43 isolates of Acinetobacter collected from samples like peripheral venous catheter tips, urine from Foley’s catheter, central venous catheter tips and endotracheal tube aspirates were preserved and processed. The tube method was performed to qualitatively detect Biofilm production. Antimicrobial susceptibility was done as per CLSI guidelines. Place of Study and Study Period: Yenepoya medical college, Mangaluru and June 2015 to December 2015. Study Design: Prospective Results: Out of 43 isolates of Acinetobacter, 26(60%) showed biofilm production, among which 19 isolates (73%) were Multidrug resistant (MDR) and only 7 isolates (27%) were Non-MDR. Highest sensitivity was seen to colistin (100%) followed by imipenem (73.7%) and least sensitivity to ampicillin (0%) followed by ciprofloxacin (3.8%). Conclusion: Antibiotic resistance was found to be significantly higher among biofilm producing Acinetobacter isolates than nonbiofilm isolates. Routine and advanced studies of the biofilm production will help in making better usage of the invasive devices with less critical complications. Keywords: Biofilms, Acinetobacter, Multidrug resistance.

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Abstract

Objective: To find out the bacteriological profile of post burn infections in wound. Place and duration of study: Vijayanagar institute of medical sciences, ballari, over a period of one year from 1st January 2014-31st December 2014. Study design: Prospective Materials and Methods: Swabs were collected from the burn wounds using standard methods and cultured for the growth of the bacteria.The organisms isolated were identified by standard microbiological methods following which antimicrobial susceptibility test was done by Kirby-baeur disk diffusion test. Results: The predominant organism isolated from swab culture was Pseudomonas aeruginosa(23%), followed by Staphylococcus aureus(22%), E.coli(14%), K.pneumoniae(9%), K.oxytoca(8%), CONS(7%), Proteus mirabilis(5%), C.freundii(4%) and C.koseri(3%),others(3%) and sterile culture(3%).The antibiogram of Pseudomonas aeruginosa showed high susceptibility to Imipenem( 81%) Piperacillin/Tazobactam (78%), Amikacin(76%), Ceftazidime(60%) and low susceptibility to Gentamicin(57%), Tobramycin(57%), Cefotaxime(47%), and Ceftriaxone(39%).Total resistance pattern was noted in Ampicillin, Cotrimoxazole, Ciprofloxacin. Keywords: Antibiotic susceptibility, Burn wounds, Pseudomonas aeruginosa.

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