Authors : Mandeep Sen, Mandeep Sen, Sana Islahi, Sana Islahi, Anupam Das, Anupam Das, Jyotsna Agarwal, Jyotsna Agarwal
DOI : 10.18231/j.ijmmtd.2019.043
Volume : 5
Issue : 4
Year : 2019
Page No : 188-192
Introduction: The bacterial profile of pus samples in many studies remain the same, but the antibiotic
resistance pattern of these isolates has shown a lot of variations.
Objective: Comparative study of bacteriological profile and antimicrobial susceptibility of Staphylococcus
aureus isolated from pus and wound swab of patients at secondary and tertiary care level centres.
Material and Methods: This observational retrospective study was conducted at the department of
Microbiology in a super specialty Post Graduate institute. Antibiotic sensitivity testing of Staphylococcal
isolates was performed by modified Kirby Bauer’s disc diffusion method as per CLSI 2017 and EUCAST
guidelines. Automated susceptibility testing was performed by Vitek-2 Compact system.
Conclusions: This study provides a comparative All medical pus specimens from both institution as well
as from SRCLI (State Referral Centre for Clinical Lab Investigation) were included of Staphylococcus
aureus isolated from pus and wound swab of patients to formulate the local antibiotic policy to initiate the
appropriate empirical antibiotic treatment at secondary and tertiary care level centres.
Results: Out of 997(59.38%) samples showing single growth, Gram positive organisms were 216 (35.23%)
for institutional and 195 (50.78%) for SRCLI (State Referral Centre for Clinical Lab Investigation). 85%
and 91% isolates of Staphylococcus aureus were resistant to penicillin followed by erythromyc in (53.5%
and 49%) and cefoperazone-sulbactum (28.5% and 14%), while 31 (55%) and 36 (34.61%) isolates were
MRSA respectively for institutional and SRCLI. Even the higher antibiotic like linezolid showed 2.5%
resistance (P = 0.5054) in MRSA isolates of SRCLI compared to 0% in institutional samples, which was,
though not statistically significant, but is of great concern. Vancomycin showed no resistance in both areas.
Keywords: Pyogenic Infections, Secondary and Tertiary Health Care, Level Centre, Antimicrobial Resistance