Authors : Valentina Y, Umadevi S, Pramodhini S
DOI : 10.18231/j.ijmmtd.2020.027
Volume : 6
Issue : 2
Year : 2020
Page No : 123-125
Aim: To compare the different phenotypic methods for detection of bacteriological profile on Vancomycin
Resistant Enterococci (VRE) among clinical isolates
Design: Prospective study
Material and Methods: 480 Clinical samples sent for bacteriological examination and culture sensitivity
were taken up in this study. Preliminary findings and identification of Enterococci species was carried out
using Grams staining, Catalase test, Bile esculin test and growth in NaCl, followed by Antibiotic sensitivity
testing in Muller Hilton Agar. The resistant strains were subjected to agar dilution method, Vancomycin estrip
method and Vitek-2 automated system for phenotypic detection of Vancomycin resistance. The results
were observed and analysed.
Results: Among 480 clinical specimens analysed, 120 Enterococci species (25.0%) were isolated. Out of
120 isolates, 40 (33.33%) were resistant to Vancomycin and 80 (66.7%) were sensitive to Vancomycin by
disc diffusion method. On further analysis, Vancomycin E-Strip and Vitek-2 showed almost similar results
making them more reliable compared to agar dilution method.
Keywords: VRE, Enterococci, Phenotypic methods, Vitek2, Estrip, Resistance.