Anti herpes simplex virus-2 activity of the extracts of Sericostoma pauciflorum stocks ex wight and Lepidagathis trinervis Nees

Authors : Boskey Pancholi

DOI : 10.18231/j.ijmr.2022.032

Volume : 9

Issue : 3

Year : 2022

Page No : 177-182

Aims: To determine the anti-HSV 2 properties and mechanism of action of two important medicinal plants Sericostoma pauciflorum Stocks exWight (Boraginaceae) and Lepidagathis trinervis Nees (Acanthaceae).
Methods and Results: To identify natural products with anti-HSV-2 activity, the aqueous and 50% ethanolic extracts were prepared from whole plants of S. pauciflorum and L. trinervis. Cytotoxicity assay using Vero cells as target revealed CC₅₀ values of these extracts ranging from 301.8 to 436.8 µg ml^-1.  Extracts from both the plants showed dose dependent inhibition of HSV-2 infection using Plaque Reduction Assay. Among the tested extracts, 50% ethanolic extract of L. trinervis showed lowest IC₅₀ value of 6.86
µg ml^-1. To study the possible mode of antiviral action, all the extracts showed 50 to 97% reduction in the formation of plaques in the attachment assay. Extracts prepared from L. trinervis also inhibited the HSV-2 penetration in a time and dose dependent manner. In post-attachment assay, both the extracts from S. pauciflorum as well as 50% ethanolic extract from L. trinervis showed significant inhibition in HSV-2 infection.
Conclusions: These studies document anti-HSV-2 activity of the two commonly used medicinal plants that will have implications in the development of an alternate remedy for HSV-2 infection.      

Keywords : Sericostoma pauciflorum, Lepidagathis trinervis, anti­ HSV­ 2 activity, Attachment assay, Penetration assay, Post­ attachment assay 3

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