Method development and validation of RP-HPLC for simultaneous estimation of cilnidipine and valsartan in synthetic mixture

Authors : Farhana V Buchiya, Hasumati A Raj, Vineet C Jain, Mihir Bhatt, Kaushik Patel

DOI : 10.18231/j.ijpca.2020.019

Volume : 7

Issue : 3

Year : 2020

Page No : 119-124

Reverse phase-high performance liquid chromatography (RP-HPLC) method have been developed and
validated for the estimation of Cilnidipine and Valsartan in bulk drug and synthetic mixture. The developed
method is rapid, accurate, precise, simple and economical. The separation was carried out using Luna
C₁₈ 100A^? (250 mm × 4.6 mm i.d.) 5 mm reverse phase column (phenomenex, luna®) in gradient mode,
with mobile phase containing Acetonitrile: Water (85:15, v/v). The flow rate is 1.0 ml/min and effluents
are monitored at 240 nm. Chromatogram showed peak at a retention time of 2.083 min for Cilnidipine
and 5.458 min for Valsartan. The method is validated for system suitability, linearity, precision, accuracy
specificity, ruggedness, robustness, LOD and LOQ. Recovery of Cilnidipine and Valsartan is found to be
100.36% and 100.14% respectively. The LOD and LOQ for estimation of Cilnidipine and Valsartan are
found to be 0.037mg/ml, 0.31mg/ml and 0.206mg/ml, 0.62mg/ml respectively. Proposed method can be
successfully applied for the quantitative determination of Cilnidipine and Valsartan in bulk drug and in
synthetic mixture.

Keywords: Cilnidipine, Valsartan, RPHPLC, Synthetic mixture.

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