Diagnostic evaluation of microscopic enteritis in duodenal biopsies of suspected malabsorption cases with clinico-immunohistological correlation

Authors : Ritika Gupta, Ritika Gupta, Sarojini Raman, Sarojini Raman, Bipad Bhanjan Mallick, Bipad Bhanjan Mallick, Urmila Senapati, Urmila Senapati

DOI : 10.18231/j.ijpo.2022.002

Volume : 9

Issue : 1

Year : 2022

Page No : 3-9

Background: Microscopic Enteritis (ME) is characterized by increase in intraepithelial lymphocytes (IEL) in intestinal mucosa. It represents a common feature of broad group of diseases including gluten mediated and non gluten related diseases. Duodenal biopsies play an important role in diagnosing these group of disorders.
Aim: To compare IEL counts in Hematoxylin and eosin (H & E) stain and CD3 immunohistochemical (IHC) stain in duodenal biopsies of suspected malabsorption cases and compare them with clinical, immunological and biochemical parameters.
Setting & Design: This was a prospective study of two years.
Materials and Methods: 164 patients were studied. IEL counted at villous tip and base in H&E sections and IHC were compared in duodenal biopsy. Data of clinical history, other parameters were collected and correlated whenever available.
Statistical Analysis: To compare any two variables, Chi - square test and independent T test was used. Statistical significance was defined as p < 0> Results: Out of 164 cases,105 cases had increased IEL. The age range was 4 to 94 years with mean age of 43.29 ± 17.96 years. Males (56, 53.3%) were affected more than females (49, 46.6%). The clinical and histological parameters showing statistical significance with raised IEL were pallor, dyspepsia, loss of appetite, crypt architecture, blunting, ulcer, villous crypt ratio, exudates, reactive atypia and edema with P< 0> Conclusion: ME should be investigated and diagnosed in correlation with a detail clinical history, complete haematological, biochemical and serological findings.

Keywords: Biopsy, Intestinal mucosa, Intraepithelial lymphocytes, Malabsorption syndromes

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