Authors : Ujjwala Supe, Nikita Mishra and Hemshankar Sahu
DOI : 10.58537/jorangrau.2025.53.1.01
Volume : 53
Issue : 1
Year : 2025
Page No : 1-10
This study investigates effective sterilization methods for the in vitro culture of Bryonialaciniosa (Linn.) in 2022-23, revealing optimal disinfection with 0.2 per cent sodium hypochloritefollowed by fungicide (Sofia) treatment. Although 0.3 per cent mercuric chloride yielded highexplant survival, it elevated culture mortality, favoring sodium hypochlorite for successfuldisinfection. An efficient protocol for callusing and regeneration via direct and indirect meanswas established using leaf segments. The different forms of callus such as compact, nodular andgreen callus were produced only multiple shoots. Better quality of pure callus was obtained in themedium fortified with MS nutrients, 3.0 mg/L 2,4-D. The induced callus produced multiple shootson the same mother medium after 3-4 weeks of culture. The induced shoots were also elongatedin the same mother medium without the addition of any other plant growth regulator. 7 shootswere regenerated in leaf explants in MS medium with KIN (4 mg/L) and IAA (1 mg/L).Elongatedshoots took roots supplemented with 4.0 mg/L KIN and 1.0 mg/L IBA. The regenerated plantswere successfully hardened into pots after proper acclimatization. These findings underscorethe potential of explants for callus induction, morphological analysis, and indirect plantletregeneration, emphasizing the importance of in vitro techniques in preserving endangeredmedicinal plants.