Diabetes Reversal Through Ayurvedic Lifestyle
PHPC Compound is the patent and proprietary
medicine developed by Benmoon Pharma Research
Pvt Ltd., Ahmedabad. The entire study was
planned to do evaluation of efficacy and safety of
PHPC Compound in experimental animal’s
models. First it was screened in-vitro using breast
cancer cell line using MTT assay method, wherein
it has shown significant reduction in viable
cancerous cells.
After promising result in in-vitro study, safety was
checked using Acute toxicity study (OECD 425),
wherein it was found safe till the dose of 2000-
5000mg/kg, orally.
Further, for long term usage of this formulation, its
sub-chronic (90 days) oral toxicity study was done
(AYUSH & OECD 408 guideline) in rats and
guinea pigs, wherein it shows no significant toxic
sign, symptoms and in histology too. To check
whether it retards toxic effects against
chemotherapeutic agents induced toxicity, it was
evaluated against 5-FU model, wherein also it has
shown chemo-preventive activity. To check its effectiveness against breast cancer, it
was further screened against DMBA-induced
cancer model, wherein also it has shown significant
reduction in occurrence of tumor, reduction in
tumor size and volume. DMBA induced breast
cancer by DNA mutation and increasing free
radicals level. The treatment of PHPC Compound
reduces free radical levels as evident by significant
increase in SOD and Catalase level. Further, it
reduces serum level of SGOT and SGPT level,
which usually get secreted during tissue damage.
Further to check the effectiveness of the PHPC
Compound on DNA damage in individual cells of
cancer cell line. In the present study, in 5-FU
(5.0μg/ml ) treated cell line, there was reduction in % Head and increase in % Tail, which indicates
DNA damage in L929 and MCF-7 cell line.
Wherein in case of Test drug (20, 40 and 60 μg
/ml) groups, there was significant increase in %
Head and decrease in % Tail, which indicates that
test drug provides chemo preventive effects. In
brief, PHPC Compound has shown promising level
of safety and efficacy in experimental animal
models.
Keywords:- cancer, cancer diagnosis, cancer
treatment, cancer research, cancer cell, oncology,
tumor, breast cancer, chemotherapy, radiation
therapy, tumor size, DNA, tumor growth, research
and development, cancer phase, metastatic,
malignanat cells
medicine developed by Benmoon Pharma Research
Pvt Ltd., Ahmedabad. The entire study was
planned to do evaluation of efficacy and safety of
PHPC Compound in experimental animal’s
models. First it was screened in-vitro using breast
cancer cell line using MTT assay method, wherein
it has shown significant reduction in viable
cancerous cells.
After promising result in in-vitro study, safety was
checked using Acute toxicity study (OECD 425),
wherein it was found safe till the dose of 2000-
5000mg/kg, orally.
Further, for long term usage of this formulation, its
sub-chronic (90 days) oral toxicity study was done
(AYUSH & OECD 408 guideline) in rats and
guinea pigs, wherein it shows no significant toxic
sign, symptoms and in histology too. To check
whether it retards toxic effects against
chemotherapeutic agents induced toxicity, it was
evaluated against 5-FU model, wherein also it has
shown chemo-preventive activity. To check its effectiveness against breast cancer, it
was further screened against DMBA-induced
cancer model, wherein also it has shown significant
reduction in occurrence of tumor, reduction in
tumor size and volume. DMBA induced breast
cancer by DNA mutation and increasing free
radicals level. The treatment of PHPC Compound
reduces free radical levels as evident by significant
increase in SOD and Catalase level. Further, it
reduces serum level of SGOT and SGPT level,
which usually get secreted during tissue damage.
Further to check the effectiveness of the PHPC
Compound on DNA damage in individual cells of
cancer cell line. In the present study, in 5-FU
(5.0μg/ml ) treated cell line, there was reduction in % Head and increase in % Tail, which indicates
DNA damage in L929 and MCF-7 cell line.
Wherein in case of Test drug (20, 40 and 60 μg
/ml) groups, there was significant increase in %
Head and decrease in % Tail, which indicates that
test drug provides chemo preventive effects. In
brief, PHPC Compound has shown promising level
of safety and efficacy in experimental animal
models.
Keywords:- cancer, cancer diagnosis, cancer
treatment, cancer research, cancer cell, oncology,
tumor, breast cancer, chemotherapy, radiation
therapy, tumor size, DNA, tumor growth, research
and development, cancer phase, metastatic,
malignanat cells