Diabetes Reversal Through Ayurvedic Lifestyle

Authors : Dr. Dinesh Kacha

DOI : 10.35629/7781-0803483486

Volume : 8

Issue : 3

Year : 2023

Page No : 483-486

PHPC Compound is the patent and proprietary medicine developed by Benmoon Pharma Research Pvt Ltd., Ahmedabad. The entire study was planned to do evaluation of efficacy and safety of PHPC Compound in experimental animal’s models. First it was screened in-vitro using breast cancer cell line using MTT assay method, wherein it has shown significant reduction in viable cancerous cells. After promising result in in-vitro study, safety was checked using Acute toxicity study (OECD 425), wherein it was found safe till the dose of 2000- 5000mg/kg, orally. Further, for long term usage of this formulation, its sub-chronic (90 days) oral toxicity study was done (AYUSH & OECD 408 guideline) in rats and guinea pigs, wherein it shows no significant toxic sign, symptoms and in histology too. To check whether it retards toxic effects against chemotherapeutic agents induced toxicity, it was evaluated against 5-FU model, wherein also it has shown chemo-preventive activity. To check its effectiveness against breast cancer, it was further screened against DMBA-induced cancer model, wherein also it has shown significant reduction in occurrence of tumor, reduction in tumor size and volume. DMBA induced breast cancer by DNA mutation and increasing free radicals level. The treatment of PHPC Compound reduces free radical levels as evident by significant increase in SOD and Catalase level. Further, it reduces serum level of SGOT and SGPT level, which usually get secreted during tissue damage. Further to check the effectiveness of the PHPC Compound on DNA damage in individual cells of cancer cell line. In the present study, in 5-FU (5.0μg/ml ) treated cell line, there was reduction in % Head and increase in % Tail, which indicates DNA damage in L929 and MCF-7 cell line. Wherein in case of Test drug (20, 40 and 60 μg /ml) groups, there was significant increase in % Head and decrease in % Tail, which indicates that test drug provides chemo preventive effects. In brief, PHPC Compound has shown promising level of safety and efficacy in experimental animal models. Keywords:- cancer, cancer diagnosis, cancer treatment, cancer research, cancer cell, oncology, tumor, breast cancer, chemotherapy, radiation therapy, tumor size, DNA, tumor growth, research and development, cancer phase, metastatic, malignanat cells


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