K Maria Kumar

Abstract

Aims and Objectives: Error analysis of the comparative study of glucose estimation with the comparison and regression analysis. Introduction: Glucose is the major carbohydrate found in the blood and a chief source of energy in human body. Estimation of glucose is very important in clinical diagnosis of hyperglycemia, hypoglycemia and normoglycemia. Accuracy of test results are very important, requires considerable effort and cost. For introducing in the laboratory the test method has to be evaluated by the process called quality control. Method selection and evaluation is important in improving efficiency. Materials and Methods: The preferred sample for Glucose assay is fasting serum or plasma. A total of 105 samples are analysed for the quantitative estimation of blood glucose by the reference method. Discussion and Results: In the present study comparison between a test method (Glucose oxidaseperoxidase) and a reference method (Hexokinase), the errors are less than TEa. Mean value of Hexokinase method is 311.36 137.315 and mean value Glucose oxidase-peroxidase method is 291.87 133.412. p-value on comparison = 0.00014. With this the test method can be an alternative to reference method in terms of accuracy and precision and analytical sensitivity and specificity. Conclusion: Glucose oxidase-peroxidase method is also a suitable method for the quantitative estimation of glucose in need of clinical requirements. Keywords: Serum glucose, Comparison and correlation, Glucose oxidase -peroxidase, Hexokinase, Method evaluation, Quality control.

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Abstract

Introduction: Thyroid diseases are not uncommon in women of reproductive age with an estimation of 5-15%. The thyroid gland must produce 50% more thyroid hormone in pregnancy in order to maintain euthyroidism and for foetal wellbeing. Aims and Objectives: To study the effect of hypothyroidism in pregnant women, attending for ante-natal check-up to the dept. of OBG – GGH, Guntur. Inclusion Criteria: Pregnant women with hypothyroidism with or without treatment, pregnant woman in euthyroid state. Exclusion Criteria: Pregnant women with hyperthyroidism, smoking alcoholism, chronic infections, systemic illness and diabetes/impaired glucose tolerance and hypertension. Materials and Methods: Singleton pregnant women aged ? 18 years TSH >2.5µIU/ml in first trimester and TSH >3µIU/ml in second and third trimester with normal T4 levels are included as cases. Controls are age and trimester matched healthy first, second and third trimester, singleton euthyroid pregnant women. Results: The present study was carried out on age matched 120 pregnant women of which 60 are hypothyroid and 60 are euthyroid. Out of 60 cases 3 cases (5%) are with overt hypothyroidism and 57 cases (95%) are with subclinical hypothyroidism. Keywords: Overt thyroid, Subclinical thyroid, TSH-Thyroid stimulating hormone, Chemiluminescence, Maternal serum sample.

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