Deepti S Ireshanavar

Abstract

Lipid profile is done in fasting blood specimen commonly, the reasons being: 1. Triglycerides remain elevated for several hours in postprandial state, 2. Reference range are established for fasting serum lipid profile. This study was proposed to compare the lipid profiles obtained in fasting and non-fasting conditions. If a reliable correlation between fasting and non-fasting lipid profile will be obtained, assay for lipids in nonfasting state may be adopted for clinical evaluation. This might help in reducing the difficulties faced in obtaining the sample in fasting state. Materials and Methods: This was a cross-sectional study, conducted from October 2011 to October 2013. Total of 200 subjects were included in the study. Two Samples were collected from each individual, one sample after 8-12 hours fasting and second sample in non-fasting state (random sample). Total cholesterol, Triglycerides, HDL-cholesterol were analyzed by standard methods and, LDL-cholesterol was calculated using Friedewald’s formula. Results: Significant increase was observed in non-fasting Triglyceride and VLDL-C as compared to fasting samples in total population, but we observed significant difference in total cholesterol and LDL-C in total population. Significant reduction was observed in HDL-C in non-fasting state as compared to fasting state. Positive correlation was observed between fasting and non-fasting total cholesterol triglyceride, VLDL-C, HDL-C and LDL-C in the total population. Conclusion: Non-fasting sample can be used to measure Total cholesterol and HDL-C. Non-fasting sample can be used to measure triglyceride, VLDL-C and LDL-C provided reference intervals are established in non-fasting state for the same in larger population. Keywords: Lipid profile, Fasting, Nonfasting, HDLC reference interval, Triglyceride LDLC, Friedewald’s formula.

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