N Asha Kiran

Abstract

Background: The abnormal levels of serum calcium and phosphorus are mostly seen in diseases of bone, kidney, tuberculosis, parathyroid diseases etc. The present study is aimed to measure the serum total calcium and phosphorous levels in HIV infected patients and in healthy controls. Materials and Methods: This cross sectional case-control study was conducted at Department of Biochemistry, NRI Medical College and General Hospital, Chinnakakani, Guntur, AndhraPradesh. A total of 90 HIV positive patients were taken as cases and 90 healthy subjects served as controls. Patients with congestive heart failure, liver diseases, diabetes and hypertension were excluded from the study. Seropositivity of all 90 HIV patients was confirmed by HIV TRI-DOT test. 5 ml of venous blood sample was collected after taking aseptic precautions from the study subjects. Serum was used for theestimation of Calciumby O-Cresolphthalein complexone method and Phosphorus by Fiske and Subbarow Method. Results: In this study, HIV positive patients had significantly reduced mean serum calcium (8.020.86 mg/dl) and phosphorous (2.680.65 mg/dl) levels compared with healthy subjects. Conclusion: The present study may conclude that serum levels of calcium and phosphorus were decreased in HIV positive patients, suggesting that the levels of calcium and phosphorous should be closely monitored. Keywords: Highly active anti-retroviral therapy, Anti-retroviral therapy, Human immunodeficiency virus, Bone mineral density.

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Abstract

Introduction: Liver disease is considered as one of the main health concern in Human Immunodeficiency Virus (HIV) patients. Diseases of the liver in HIV patients encompass spectrum of liver abnormalities. Elevated levels of liver enzymes like aspartate transaminase (AST), alanine transaminase (ALT) and alkaline Phosphatase (ALP) are markers of hepatocyte injury. The aim of the present study was to evaluate the clinical significance of liver enzymes such as AST, ALT and ALP in HIV patients on antiretroviral therapy. Materials and Methods: In this cross sectional case-control study, a total of 180 subjects were included in this study. Among them 90 HIV positive patients were included as cases and 90 normal subjects were included as controls. Seropositivity of all 90 HIV patients was confirmed by HIV TRI-DOT test. Estimation of serum AST, ALT and ALP was done by using automated chemistry analyzer. P value Results: In this study, there is a significant elevation in liver enzymes such as AST, ALT and ALP (p Conclusion: In this study, we conclude that, significantly elevated levels of liver enzymes is observed in HIV positive patients under the treatment. Therefore, the elevated levels of liver enzymes should be analyzed and monitored in HIV patients to prevent further progression of disease. Keywords: CD4+ cells, Liver disease, HIV, Hepatotoxicity, Anti-retroviral therapy.

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