Vijetha Shenoy Belle

Abstract

Introduction: Diabetes mellitus, the most common chronic metabolic disorder affecting about 300 million people all over the world. It occurs due to impaired insulin secretion, impaired insulin action or both whereas Prediabetes is the initial stage before diabetes mellitus and characterized by impaired fasting glucose or impaired glucose tolerance. Type 2 diabetes mellitus is associated with increased metabolic processes and oxidative stress. The trace elements are important co-factor in these events. Thus, this study was conducted to compare serum copper, zinc in prediabetes, diabetics with normal controls. Materials and Methods: Institutional ethics committee permission was obtained prior to the study. Study included 71 prediabetes, 71 diabetics subjects and 71 controls between 40-60 years. Serum fasting blood sugar, glycated hemoglobin, copper, zinc was estimated. Statistical analysis was done using student t-test and Pearson’s correlation. p value Results: Serum copper was significantly increased in diabetic (77.87+40.78) and serum zinc (128.05+20.87) was significantly decreased in diabetics compared to apparently healthy controls. (Copper =60.72+19.70, zinc =140.74+33.99), where as in prediabetics both copper and zinc level were increased compared to healthy subjects. Serum copper was positively correlated with Glycated hemoglobin (r=0.26, p= Glycated hemoglobin (r= - 0.182, p=0.008) and fasting blood sugar (r= - 0.188, p=0.006). Copper to zinc ratio also showed statistically significant positive correlation with Glycated hemoglobin (r=0.287, p= ) Conclusion: Serum copper and zinc have a role in diabetes thus supplementation of micronutrients may be essential to maintain the diabetic status. Keywords: Diabetes mellitus, Zinc, Copper, Copper to zinc ratio.

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Abstract

Introduction: Ischemia is a worldwide scare and denotes decreased oxygen supply to various tissues of our body. Ischemia modified albumin, a biomarker to diagnose cardiac ischemia has a role in oxidative stress. Diabetes mellitus carbohydrate metabolic disorder is an oxidative stress condition. Total Thiol is an antioxidant, which reduces the reactive oxygen species. There have been lot of research which proved the association of diabetes with ischemia modified albumin. In this study we tried to compare the association of ischemia modified albumin with prediabetes as well as diabetes. Materials and Methods: After obtaining the ethical approval from institutional ethics committee, 120 serum samples were collected (40 - normal healthy subjects, 40 - prediabetes, 40 - diabetes mellitus) and analyzed for ischemia modified albumin using spectrophotometric Co(II)-albumin binding assay and total Thiol was measured using spectrophotometric method. Glycated hemoglobin, lipid profile values were obtained from laboratory information system. Data were compiled and appropriate statistical tests were used wherever necessary. p value Result: This study reported decreased levels of ischemia modified albumin and increased levels of total thiol in both prediabetes as well as diabetes mellitus as compared to normal control. Significant positive correlation between ischemia modified albumin and total thiol was obtained. Conclusion: Oxidative stress maker IMA can be neutralized by an anti-oxidant total thiol. People consume antioxidant to prevent progression of chronic diseases. Extra supplementation of antioxidant may not be much helpful in cases where naturally antioxidants are produced due to the diseases. Thus measuring both helps in prevention of extra supplementation of anti-oxidants. Keywords: Ischemia modified albumin, Total thiol, Prediabetes, Diabetes mellitus, Lipid profile, Glycated hemoglobin.

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