Mohammad Shahnaz

Abstract

Agaricus bisporus, being one of the most commonly white button mushrooms contributes about 40- 45% to the world mushroom production. Presence of viable cells were measured in terms of viable count or CFU, which exhibit progressive declination in count of thermophilic fungi from initial stage of composting. Similarly, declination of pH ranges from 8.3-7.1 alkaline pH in initial stage was due to microbial decomposition of organic acids and subsequent release of ammonia through mineralization of organic nitrogen sources, declination of weight from 1.465 Kg. to 0.491 Kg and temperature from 44-34?C was due to extensive utilization of compost as nutrient by inhabiting thermophilic fungi. Result obtained exhibit highest cellulose content in raw material of which horse manure exhibit higher cellulose content then wheat straw, horse manure 2.052mg and wheat straw 1.044mg. Highest cellulolytic activity was observed in horse manure followed by wheat straw in basal material. Horse manure 2.167micromoleglucose released/ml/hr and wheat straw 1.014micromoleglucose released/ml/hr. Highest antimicrobial activity was observed in methanol extract (14mm) against B. cereusas compared to ethanol extract (7mm). Same results obtained for S.aureus(20mm) and B. cereus (14mm) in methanol extract. Least microbial growth inhibition was observed in S. aureus (25mm) followed by B. cereus (7mm) with respect to control in ethanol extract. No antimicrobial activity observed in P. aeruginosa in ethanol but least inhibition was observed in methanol extract. Keywords: Agaricus bisporus, White button mushrooms, Thermophilic fungi, Antimicrobial activity.

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Abstract

Precision: The degree of agreement among individual test results when a method is applied to multiple samplings of a homogeneous sample. It is a measure of either the degree of reproducibility (agreement under different conditions) or of repeatability (agreement under same condition) of the method. Linearity: The ability of a method to produce results that is directly or indirectly proportional to the conc. of the analyte in samples within a given range. Range: The interval between upper and lower level of analyte (including those levels) that has been shown to be determined with precision, accuracy and linearity using the method as written. Accuracy: The closeness of test results obtained by method to the true value. It is a measure of the exactness of the method. Ruggedness: The ruggedness of an analytical method is the degree of reproducibility of test results obtained by analysis of the same samples under a variety of normal test conditions. Such as different laboratories, different analyst, different instruments, different lots of reagents different elapsed assay times, differently days at normal lab. Conditions etc. Intermediate precision is normally expressed as the lack of influence on test results of operational and environmental variables of the analytical method. Ultraviolet Visible spectrometric assay developed for the quantification of Ketoprofen was performed in methanol in the concentration of 10 mcgml. Single Point Standardization method was used for the quantitative analysis of drug. The drug obeys Lambert – Beer’s law in the concentration range of 5 mcgml. The absorbance maxima occur at 256 nm. The developed method was validated as per ICH norms. Single Point Standardization method involves simple calculations. The absorbance value at 256 nm was found to be around 0.291. The results obtained on the validation parameters of developed method meets the ICH requirements. It infers that the method was found to be simple, specific, precise, accurate, reproducible, reliable, linear and proportional (i.e.) it follows Lambert-Beer’s Law. The method was found to be rapid and economic. Hence it can be inferred that the above method was useful to be applied in routine laboratory analysis with a high degree of accuracy and precision. Keywords: Ketoprofen, Analytical method development, Accuracy, Precision, Ruggedness, Robustness.

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