Sushant Joshi

Abstract

Background: Otomycosis is one of the most common forms of otitis externa. It is often seen in cases of Chronic Suppurative Otitis Media. It is important to treat otomycosis effectively in cases of CSOM as both conditions worsen each other. Objectives: To study the aetiology of otomycosis and fungal profile in patients of diagnosed CSOM at ENT Clinic, Udaipur. Materials & Methods: A total of 100 clinically diagnosed patients of CSOM were enrolled in the study and the samples were obtained from each patient using sterile cotton swab and were studied for fungal profile. Results: The most common fungi found in CSOM are Candida and Aspergillus species. In the present study out of 95 culture positive cases of CSOM, fungi were found in 13 cases (13.68%). Candida species was most common found in 10.52% of all cases and Aspergillus was observed in 3.15% of all cases. Out of all candida species 60% were albicans. Keywords: Otomycosis, Chronic suppurative otitis media.

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Abstract

Introduction: Contamination of operation theatres and Intensive care units through air, surface or article vehicle is major cause of surgical site and hospital acquired infections leading to increased morbidity and mortality. Aims and Objective: The present study was conducted with aim to isolate and identify the nature of microbial contamination through air, surface and equipment of OT in a tertiary care teaching hospital. Materials and Methods: Six months (August 2019 – January 2020) retrospective analysis of data by random sampling of the six OT’s of the hospital was done. Air sampling was done by settle plate method. Surface samples were taken from different sites and equipments from the OT. Bacterial species were isolated and identified by conventional methods. Results: Total 300 samples were collected and analysed Out of which 116 samples (38.66%) were positive for bacterial growth. The predominant bacterial species isolated from surface or equipment was Bacillus (48.27%), followed by Coagulase Negative Staphylococcus (21.55%), Gram Negative bacilli i.e. Klebsiella (8.62%) and least commonly isolated bacterial species was Staphylococcus aureus 2(1.72%). Air quality analysis showed highest bacterial CFU rate of 75cfu/m3 from general surgical operation theatres and Ophthalmology OT recorded least bacterial cfu rate of 20cfu/m3. Most common isolated species was Baciilus followed by Coagulase Negative Staphylococcii. Conclusion: The study revealed that the OT’s of our hospital showed a low bacterial contamination rate on surface swabbing and air quality CFU count per mm3 were within permissible limits. The present study emphasizes the need for periodic surveillance of OT’s and ICU’s and highlight the importance of early detection of bacterial contamination levels and prevention of hospital acquired infections. Keywords: Air sampling, OT, Settle plate method, Surveillance.

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