Sarmila Sen

Abstract

Background: Ovarian cancer represents 30% of all cancers of female genital tract. An accurate histopathological diagnosis of ovarian tumours is essential for their management and the outcome of therapy. Aims and Objectives: Clinicopathological study of ovarian epithelial neoplasms and to study the expression of p53 and Her2/neu in epithelial carcinoma of ovary and their association with the histological grade. Settings and Design: An observational descriptive type of study in a tertiary care hospital in Eastern India. Materials and Methods: 72 cases diagnosed as ovarian epithelial neoplasms in the department of Pathology, CNMC&H Kolkata during the study period of January, 2019 to December, 2020 were studied. Specimens were processed routinely. Immunohistochemistry was done following standard protocol. Statistical analysis: Statistical analysis was done using SPSS (version 21.0, Chicago inc., Chicago, Illinois, USA) software. Results: 57 cases were benign followed by 14 malignant and 1 borderline case. Majority of the tumours were serous type (71.4%) followed by mucinous (27.2%) and seromucinous (1.4%). P53 expression was noted in 50% of the epithelial ovarian carcinoma and 85.7% of the high grade serous carcinoma. Her2/neu expression was noted in 21.4% of epithelial ovarian carcinoma cases. Conclusion: p53 expression was more in serous carcinoma and the expression of p53 was associated with higher grade of serous carcinoma. Her2/neu expression was more in serous carcinoma. There was no significant association between Her2/neu expression and histological grade and a larger population based study is needed for further evaluation.   Keywords: Ovarian epithelial neoplasms, Her2/neu expression, p53 expression in epithelial carcinoma of ovary.

View Article

Abstract

Background: Urothelial carcinomas are the fourth most common tumors worldwide. Studies have shown that Her2/neu is associated with increased tumour grade and Ki?67 is related to tumour recurrence and stage progression. As no such study has been undertaken is this region, the present study was aimed to establish Her2/neu and Ki-67 as novel prognostic markers of urothelial neoplasms. Aims and Objectives: To evaluate the clinical profile of urothelial neoplasms and correlate the expression of Her2/neu and Ki-67 in urothelial carcinomas. Settings and Design: Observational and cross-sectional study (January 2018-May 2019) in a tertiary care hospital in Eastern India. Materials and Methods: 40 specimens of urothelial neoplasms were studied. Clinical history of patients was collected. Microscopic examination was done to assess tumour stage and histological grade. Immunohistochemistry with Her2/neu and Ki-67 was performed. Analysis: Statistical Package for the Social Sciences SPSS (version 20.0, IBM, Chicago, IL, USA) for windows software. Results: 8 cases were diagnosed as low grade non-invasive urothelial carcinoma, 31 cases as infiltrating urothelial carcinoma and 1 case as papillary urothelial neoplasm of low malignant potential (PUNLMP). 96.8% of infiltrating carcinomas and 87.5% of low grade non-invasive carcinomas showed Ki-67 positivity. Moderate-to-strong Her2/neu over[removed]2+ or 3+) was observed in only 21% of the cases. Conclusion: The expression of Ki-67 increased with increase in the grade of the tumour which was suggestive of the prognostic importance of Ki-67. Her2/neu positivity was seen in infiltrating cases. No significant association between the tumor grade and Her2/neu expression, indicated the need for further studies with a larger population group. Keywords: Her2/neu, Ki- 67, Urothelial neoplasms.

View Article