Authors : Pooja Patel, Hiral K Patel, Alka B Nerurkar
DOI : 10.18231/2394-5478.2019.0015
Volume : 6
Issue : 1
Year : 2019
Page No : 71-77
Introduction: A surgical site infections (SSI) are a 3rd most common nosocomial infection and is responsible for morbidity, mortality and increased healthcare costs.
Aims and Objectives: The main aim of this study is to identify the isolates causing surgical site infections and its anti-microbial susceptibility pattern.
Materials and Methods: A total 50 cases of surgical site infections were taken for the study. The suspected samples were processed by using standard microbiological methods. The modified Kirby-Bauer’s disc diffusion method was used for antibiotic susceptibility pattern. The isolates of Enterobacteriaceae family were initially screened for ESBL production and were further confirmed by Double Disk Synergy Test as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Reference strains of E. coli (ATCC 25922), P. aeruginosa (ATCC-27853), S. aureus (ATCC 25923) and Klebsiella 700603 were used as controls.
Result: Out of 50 samples, 19(38%) were culture positive and total 23 organisms were isolated. Pseudomonas aeruginosa 9 (39.13%) was the most common organism isolated, followed by Klebsiella spp. 5 (21.73%), Staphylococcus aureus 3 (13.04%). Most of the Gram-negative isolates were sensitive to imipenem and meropenem followed by piperacillin-tazobactam. Gram-positive organisms were found to be more sensitive to levofloxacin, linezolid and vancomycin. ESBL production was seen among 44.44% isolates of Enterobacteriaceae family.
Conclusion: The rate of infection reflects on patient's care and standard of treatment in any hospital. Thus, better stewardship of SSI is required with accurate antibiotic policies. Periodic monitoring of etiology and antimicrobial susceptibility in the community and hospital settings is recommended.
Keywords: Surgical site infection, Bacteriological profile, Antibiotic susceptibility test, ESBL (Extended Spectrum Beta-Lactamase).