Authors : Manmeet Kaur Gill, Ashish Khanna
DOI : 10.18231/j.ijmmtd.2019.036
Volume : 5
Issue : 3
Year : 2019
Page No : 163-165
Introduction: The wounds infection remains a medical problem worldwide due to associated morbidity, mortality and economic burden. Wounds can be infected with bacteria or yeast and the source is usually hosts normal flora and/or the hospital environment. Since Pseudomonas aeroginosa is the most common gram-negative non fermenter opportunistic bacteria associated with nosocomial infections. The present study was undertaken to know the antibiotic susceptibility patterns of this pathogen.
Materials and Methods: It was a cross-sectional study carried out over a period of one year. Pus samples collected at source were processed as per standard bacteriological techniques for aerobic cultures. They were inoculated on 5% Blood agar and Mac-Conkey’s agar and incubated aerobically for 24-48 hours at 37 degree before reporting them as sterile. Anaerobic cultures were not done. Non lactose fermenting colonies on MacConkey agar were subjected to oxidase test. These oxidase positive isolates were further identified by Vitek-2 Compact (Biomerieux) using gram negative identification cards, following manufacturer’s guidelines. Antimicrobial sensitivity was also determined by same system using 281 AST cards. Antibiotic sensitivity results were interpreted as per CLSI guidelines.
Results: A total of 1566 wound swab/pus samples from patients with infected wounds were processed for culture and sensitivity testing from January 2018 to November 2018.
The present study comprised of 101 isolates of Pseudomonas aeroginosa. Isolation rate was highest in the age group 20-40 years followed by those above 60 years of age. Most of the isolates obtained were multi-drug resistant. Highest sensitivity was seen in Amikacin and Imipenem.
Conclusion: In conclusion, Pseudomonas aeroginosa which is a common Gram negative bacteria isolated from wound infections and also highly resistant to different types of antibiotics could be a reason for high morbidity and mortality in hospitalised patients. Therefore a regular surveillance of antibiotic resistance of these organisms is needed to prevent indiscriminate use of antibiotics leading to emergence of drug resistance among these pathogens.
Keywords: Pseudomonas aeroginosa, Nosocomial infections, Multi-drug resistant.