Authors : Afreen Hasan, Amit Kumar, Vishnu Kumar Awasthi
DOI : 10.18231/j.ijcbr.2020.001
Volume : 7
Issue : 1
Year : 2020
Page No : 1-7
Introduction and Aims: Primary open angle glaucoma is a chronic optic neuropathy with possible
involvement of oxidative stress in its pathogenesis. The present study was conducted to evaluate the role
of oxidative stress in terms of lipid peroxide (LPO) and the protective role of antioxidant enzymes catalase
(CAT), superoxide-dismutase (SOD) and glutathione peroxidase (GPX) in patients of primary open angle
glaucoma.
Materials and Methods: A total of 120 patients were enrolled. After taking informed consent, 5 ml of
venous blood was taken under aseptic conditions in EDTA tube. These samples were transported from Eye
OPD to Laboratory in icebox maintaining temperature for enzymatic activity of enzymes in blood samples.
The blood was centrifuged, plasma was separated and was used for estimation of lipid peroxide (LPO).
RBC lysates were prepared for estimation of antioxidant enzymes namely; catalase (CAT), superoxide
dismutase (SOD), glutathione peroxidase (GPx).
Results: A total of 120 patients were enrolled. There was no significant difference in LPO levels of POAG
patients and controls. No statistically significant difference was observed in the levels of SOD and catalase.
There was a significantly raised value of GPx in POAG patients but since SOD activity was normal, we can
conclude that the SOD level was sufficient to meet the above exigency.
Conclusions: Oxidative stress is not the only cause for development of primary open angle glaucoma.
Keywords: Oxidative stress, Lipid peroxidase, Catalase, Superoxide dismutase, Glutathione peroxidase.