Authors : Marina Ramsis Fahmy, Youssry El-Sayed Saleh, Mary Sobhy Khalil
DOI : 10.18231/j.ijmr.2020.018
Volume : 7
Issue : 1
Year : 2020
Page No : 83-90
Introduction: Burkholderia cepacia complex (BCC) have recently received a reasonable attention as one
of the major risks in susceptible pharmaceutical products. This microorganism can easily spread and cause
severe contamination especially to the water stations for pharmaceutical companies. It rapidly grow within
the product and cause cystic fibrosis and septicemia in humans.
Aim: The traceability of the sources of contamination of pharmaceutical non-sterile aqueous preserved
products and how to control it during the manufacturing operations as it is concern with the public health.
Materials and Methods: The samples collected for the analysis were taken from different sources provided
from the pharmaceutical company such as: aqueous finished products, filling machines, preparation tanks
and water stations, identified by API 20NE, Vitek 2 compact system and 16S rRNA.
Results: From 213 different samples of finished product, 22 bacterial isolates had been identified as
Burkholderia cepacia group. Raw materials and primary packaging materials did not result in any bacterial
isolates while the machine surfaces and preparation tanks were contaminated. From 384 pharmaceutical
water samples, 35 isolates were identified as B.cepacia.
Conclusion: Our study suggests that the chlorine treatment and hydrogen peroxide have a significant effect
on B.cepacia in water disinfection.
Keywords: Burkholderia cepacia, Susceptible pharmaceutical products, Water treatment, Hydrogen peroxide chlorine.