Authors : Aishwarya Thakre, Rupali Maheshgauri, Manvi Aggarwal, Priyanka Mantri, Asim Naik
DOI : 10.18231/j.ijooo.2020.008
Volume : 6
Issue : 1
Year : 2020
Page No : 42-47
Purpose: To study the role of Impression cytology in various ocular surface disorders and to evaluate its
safety and compatability.
Materials and Methodology: A case control study was done of which 25 patients who had clinically
obvious conjunctival.
Involvement served as cases and 25 who were without any symptoms served as control. All the patients
were subjected to tests of dry eye evaluation such as Schirmers test and Tear Film Break Up time (TFBUT)
test. Conjunctival impressions were obtained using millipore cellulose acetate paper strips. Cytological
samples were graded according to Nelson and Wright classification.
Results: Patients with bitots spot had large goblet cells and squamous metaplasia. All of the patients
showed Grade 1 cytological changes (34.61% of all samples) Patients with severe chemical burns showed
Grade III cytological changes i.e 100% of samples and patient with mild chemical burn showed grade II
cytological change, i.e 99% of samples.
Conclusion: Impression cytology is a simple and non-surgical technique for diagnosis and therapeutic
control of ocular surface disorders. It helps in identifying those at imminent risk of developing symptoms
but also helps in assessing the severity of cytological changes in symptomatic subjects.
Keywords:
Cellulose acetate paper, Impression cytology, Ocular surface disorders.