Authors : , Vinod Kumar Kondreddy, Rishav, , D. R. Nagesh, , Deepika Saini, , C. Kavitha, , Mrinal Kanti Bhoumik, , Reena Sharma, , Ramanlal N. Kachave, , M. Vishala
DOI : 10.51470/bca.2025.25.2.1987
Volume : 25
Issue : 2
Year : 2025
Page No : 1987-1993
The objective of this research is to create and validate an analytical method for the RP-HPLC estimation of telmisartan and hydrochlorothiazide. The HPLC Binary Gradient System (HPLC 3000 series) was used to construct the analytical procedure. The column used was the Cosmosil C18, which had dimensions of 250mm x 4.ID and a particle size of 5µm. The mobile phase consisted of a 60:40 mixture of methanol and water, and the flow rate was 0.8 ml. The detected wavelength was 231nm. The half-life of hydrochlorothiazide was 5.5 minutes and that of telmisartan was 4.1 minutes. Two drugs, telmisartan and hydrochlorothiazide, were determined to have linearities of 0.998 and 0.999, respectively. The % recovery for Hydrochlorothiazide was 99.71% and the limit of detection for Telmisartan was 0.174, whereas for Hydrochlorothiazide it was 0.339. This method was created with accuracy, simplicity, precision and cost-effectiveness in mind. The approach provides precise estimations of hydrochlorothiazide and telmisartan.