IDENTIFICATION AND SPECIATION OF CANDIDA ISOLATED FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL

Authors : Ngairangbam Gopeshwor, Tarana Sarwat, Dalip K Kakru and Saheli Bhadra

DOI : 10.24327/ijcar.2022.1327.0296

Volume : 11

Issue : 7

Year : 2022

Page No : 1324-1327

Infections due to Candida species have increased substantially over a few decades due to increased use of broad-spectrum antibiotics, immune-suppressants and indwelling devices which attribute to high rates of invasive candidiasis. Candida albicans majority of superficial and systemic infections. Both,Candida albicans associated with high morbidity and mortality because of the increasing antifungal resistance. Hence, it is important to correctly identify and speciate them. The present study was therefore conducted to identify various species ofCandida specimens. The study was conducted over a period of one year from November 2020 to November 2021. The various species of Candida isolated, were identified by using conventional tests (germ tube and chlamydospore test, sugar fermentation and assimilation tests and commercially available CHROM agar)and automated methods (Vitek 2 Compact system). A total of 79 strains of Candida were isolated during the study period. tropicaliswas the most frequently isolated species, 41(51.8%), followed by albicans,16(20.2%), Candida krusei,11 (13.9%),Candida glabrata, dubliniensis,3(3.7%). Early identification and reporting of infections due to Candida help the clinicians in better patient management. Now a days, a wide range of molecular techniques have been used including non-DNA-based methods and DNA because they generate unambiguous and highly reproducible typing data. CHROM agar is a conventional and rapid method of identification of Candida settings, hence its use may help in making an early diagnosis.


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