Authors : Vikas Sharma, Arti Heer, Navneet Kour, Shivangi Sharma
DOI : 10.18231/j.ijpca.2023.034
Volume : 10
Issue : 3
Year : 2023
Page No : 205-208
The present study was carried out to evaluate antibacterial potential of methanolic extract from amla leaves via agar-well diffusion method against two gram positive ( subtilis MTCC2389, MTCC7443) and three gram negative (MTCC4821, MTCC2127, MTCC7162) strains. Susceptibility of plant extract was tested by serial microdilution method (MIC) and agar well diffusion method was determined. The extract showed maximum zone of inhibition against (17.6 mm) followed by (16.5 mm) and (16.2 mm). However, activity was also reported against (15.5 mm) and (15 mm). The different concentrations of extract (10?l and 20?l) were used and the minimum inhibitory concentration (MIC) ranging from 62.5 to 2000 ?g/ml was compared with the standard drug Chloramphenicol (5?g). The MIC of methanolic extract was found 550 ?g/ml against and The results of present study revealed that leaves of amla possesses antibacterial potential and source of new antibiotics. Therefore, medicinal plants are finding their way into pharmaceuticals, neutralceuticals and food Supplements that could be useful in chemotherapy to control infectious diseases.
Keywords: Amla leaves, Agar well diffusion method, Antibacterial, Methanolic extract