Authors : Praveen Sekar, Vignesh Natarajan, Nivetha Shanmugam, Thilagasundari Kandasamy
DOI : 10.18231/j.ijpca.2023.022
Volume : 10
Issue : 2
Year : 2023
Page No : 125-132
Infectious microbes like bacteria, viruses, or fungi commonly cause inflammation when they enter the body, live in certain tissues or circulate in the circulation. For long years ago, natural sources have been used for the treatment of various diseases. In this way, belonging to the family Lamiaceae was found to be having various pharmacological activities like anticancer, antimicrobial, antioxidant, anti-inflammatory, anti-diabetic, larvicidal and cardiovascular activities. The objective of the study is to formulate and evaluate the anti-inflammatory potential of the topical gel using an Ethanolic leaves extract of The formulated gel F3 showed better spreadability with a net content of 99 % and a drug release of 94.84 %. The anti-inflammatory potential of the formulation F3 was evaluated using the inhibition of albumin denaturation assay and Human red blood cells (HRBC) membrane stabilization assay. The formulated gel and standard exhibited 45.86-82.32 % and 55.25-88.07 % of inhibition at the concentration of 50-250 µg/ml, respectively at albumin denaturation assay and 25.35-75.49 % and 30.70-87.18 % of inhibition at 50-250 µg/ml concentration, respectively. Thus, the formulated topical gel using an Ethanolic leaves extract of was found to be possessing potent anti-inflammatory activity.
Keywords: Coleus amboinicus, Formulation, Ethanolic extract, Drug release, In vitro antiinflammatory activity, Phytochemical Screening