Authors : Vijayakumar C N, Sandeep Tripathi, Surabhi Tomar Sharma
DOI : 10.18231/2394-2754.2018.0027
Volume : 5
Issue : 1
Year : 0
Page No : 124-128
Aim: To evaluate the efficiency of Discontinuous Double Density Gradient Sperm Preparation over Swim up preparation in concentrating normomorphic spermatozoa in the yield.
Materials and Methods: 50 semen samples obtained from patients visiting the NIMS Fertility and Research Center were analyzed for normal morphology prior to processing, then the sample was aliquoted in to two equal halves, each of it was processed by Swim up and Discontinuous Density Gradient preparation simultaneously. Yields obtained from the both methods were tested for normal morphology of sperms using Diff quick staining method and micrometry according to WHO Manual 2010.
Results: Analyzed neat semen samples showed mean Sperm concentration of 38.2 m/ml and mean sperm morphology of 7.54%. Post processing yield from Swim up preparation had mean concentration of 30.08 m/ml and normal morphology of 8.66% (p-value 0.05) where as the yield from discontinuous density Gradient preparation showed up a mean concentration of 32.2 m/ml and normal morphology of 9.8% (p-value 0.001)
Conclusion: From the study it was evident that the Discontinuous Density Gradient Sperm Preparation method has a clear edge over Swim up preparation in concentrating normal morphological spermatozoa in the yield.
Keywords: Sperm Processing, Normal morphology, Swim up technique, Density gradient technique.